Cao, Qi published the artcileMechanism research on CSE restraining macrophages into foam cells by calcium sensing receptor, Quality Control of 226878-01-9, the publication is Xiandai Yufang Yixue (2015), 42(19), 3555-3559, 3596, database is CAplus.
Objective To confirm calcium sensing receptor (CaR) can increase the expression of CSE and the secretion of H2S, thereby inhibiting the transformation of macrophages into foam cells. Methods Sensitive sulfur electrodes method was used to detect the change of the H2S content in macrophages. The relative content of pos. cells was tested by oil red O staining and the HPLC method was sued to do the determination of intracellular cholesterol content. ELISA assay detected the secretion situation of cytokine IL-10, MIF, and TNF-α. The expression of CaR, CSE, CD36 and ACAT-1 in each group cells were detected by Western blot. Results Compared with the blank control group, the relative contents of H2S in the GdCl3 group and the NaHS group significantly increased, while the NPS2390 group, the relative content of H2S significantly decreased. The number of pos. cells in the GdCl3 group and the NaHS group obviously decreased, while the number in NPS2390 group adversely increased. The levels of TNF-α and MIF in cell supernatant significantly ascended, and the IL-10 level fell in the GdCl3 and NaHS groups. However, there was a rising trend on the levels of TNF-α and MIF in cell supernatant, and the IL-10 level dropped significantly in the NPS2390 group. The expression of CaR and CSE significantly enhanced in GdCl3 group, while the expression of CD36 and ACAT-1 were significantly inhibited in GdCl3 group. Meanwhile, the expressions of CaR and CSE were significantly inhibited in NPS2390 group, while the expression of CD36 and ACAT-1 were significantly enhanced in NPS2390 group. GdCl3 group significantly increased the expression of CSE, while GdCl3 + CSE siRNA group and CSE siRNA group significantly reduced the expression of CSE. GdCl3 group significantly increased the relative content of H2S, while GdCl3 + CSE siRNA group and CSE siRNA group significantly reduced the relative content of H2S. Conclusion CaR could enhance the expression of CSE in macrophages to increase the secretion of H2S, thereby inhibiting the transformation of macrophages into foam cells.
Xiandai Yufang Yixue published new progress about 226878-01-9. 226878-01-9 belongs to quinoxaline, auxiliary class Neuronal Signaling,mGluR, name is N-(Adamantan-1-yl)quinoxaline-2-carboxamide, and the molecular formula is C19H21N3O, Quality Control of 226878-01-9.
Referemce:
https://en.wikipedia.org/wiki/Quinoxaline,
Quinoxaline | C8H6N2 | ChemSpider