A novel near-infrared fluorescent probe for rapid detection of peroxynitrite with large stokes shift and imaging in living cells was written by Parthiban, C.;Manivannan, R.;Son, Young-A.. And the article was included in Journal of Photochemistry and Photobiology, A: Chemistry in 2022.Name: 6-Methoxyquinoxaline This article mentions the following:
Peroxynitrite (ONOO–) plays an essential role in pathol. and physiol. processes like signal transduction, antibacterial activity, etc. Imagining ONOO- in the living system is more important to monitor their function. We have analyzed various coumarin derivatives using DFT computations. We have designed and synthesized a coumarin chalcone-hybrid (Coumn-pHP-ONOO–) based turn-on NIR fluorescent probe for the rapid detection of ONOO– based on oxidative hydrolysis of aryl boronate group in a living system. Our probe Coumn-pHP-ONOO– shows sensitive and selective detection of ONOO– in the presence of various reactive species with a large stokes shift (149 nm) in THF/Water (6/4, volume/volume, pH = 7.4) solution and rapid response (<5s). The detection limit of the probe is 53.8 nM level and has a reasonable specificity in the presence of other reactive oxygen species. Moreover, our probe Coumn-pHP-ONOO– displays low toxicity, good biocompatibility, excellent permeability, and it successfully detects the ONOO– in HeLa cell. In the experiment, the researchers used many compounds, for example, 6-Methoxyquinoxaline (cas: 6639-82-3Name: 6-Methoxyquinoxaline).
6-Methoxyquinoxaline (cas: 6639-82-3) belongs to quinoxaline derivatives. Quinoxaline is isomeric with other naphthyridines including quinazoline, phthalazine and cinnoline. Quinoxalines are used in the treatment of bacterial, cancer, and HIV infections. Moreover, varenicline, a clinical drug is used for treating nicotine addiction, also contains quinoxaline moiety.Name: 6-Methoxyquinoxaline
Referemce:
Quinoxaline – Wikipedia,
Quinoxaline | C8H6N2 | ChemSpider