Cleaning method development and validation of brimonidine tartrate residue from brimonidine tartrate API was written by Ali, Sayyad. Kousr;Mandal, Tanmoy. And the article was included in Analytical Chemistry: An Indian Journal in 2021.Electric Literature of C15H16BrN5O6 This article mentions the following:
To Develop the Precise, Linear and Accurate Cleaning method Validation for the Brimonidine Tartrate from Brimonide tartrate Active Pharmaceutical Ingredient as per International Conference on Harmonization guidelines by using High Performance Liquid Chromatog. In this method uses a reverse phase C18 column make as inert sustain AQ C18 column (250 x 4.6 mm, 5μ), mobile phase-a as 0.1% ortho phosphoric acid and mobile phase-b as acetonitrile : methanol (50:50) volume/volume, flow rate as 1.2 mL/min, and detection of wavelength AS 248 nm, column temperature 40°C, injection volume 10μL and autosampler temperature 25°C by using Photo Diode Array Detector. In this method Brimonidine tartrate peak was eluted at 3.9 min. Brimonidine tartrate linear range was 0.5μg/mL to7.5μg/mL. In System suitability the % Relative standard deviation observed as 0.5. In swab and Rinse samples recovery was found between 97% to 103%. The limit of Quantification and limit of Detection range is found to be 0.5μg/mL and 0.15μg/mL resp. An accurate, linear and precise Reverse phase High Performance Liquid Chromatog. method was developed and validated as per International Conference on Harmonization guidelines. Hence this cleaning method validation is used for routine anal. of the residue samples in various pharmaceutical manufacturing areas. In the experiment, the researchers used many compounds, for example, 5-Bromo-N-(4,5-dihydro-1H-imidazol-2-yl)quinoxalin-6-amine (2R,3R)-2,3-dihydroxysuccinate (cas: 70359-46-5Electric Literature of C15H16BrN5O6).
5-Bromo-N-(4,5-dihydro-1H-imidazol-2-yl)quinoxalin-6-amine (2R,3R)-2,3-dihydroxysuccinate (cas: 70359-46-5) belongs to quinoxaline derivatives. Quinoxalines have received a significant amount of attention due to their potential use in fighting various pathophysiological conditions like epilepsy, Parkinson’s, and Alzheimer’s diseases. Quinoxalines are used in the treatment of bacterial, cancer, and HIV infections. Moreover, varenicline, a clinical drug is used for treating nicotine addiction, also contains quinoxaline moiety.Electric Literature of C15H16BrN5O6
Referemce:
Quinoxaline – Wikipedia,
Quinoxaline | C8H6N2 | ChemSpider